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Table 2 Summary of nanopore sequence data bearing on the fate of transgenes from four A. castellanii transformants

From: The fate of artificial transgenes in Acanthamoeba castellanii

Clone

Plasmid topology

Estimatedgenome coverage

Inferred integrations

With 2 reads

With > 2 reads

# of artefactual chimeras

1-telomere reads

2-telomere reads

C1

circular

100X

1 251

25

1

936

288

3

LT6

linear

50X

280

0

0

336

85

3

LT8

linear

100X

187

2

0

438

32

1

LT9

linear

65X

109

1

0

185

45

0