Fig. 1
From: The fate of artificial transgenes in Acanthamoeba castellanii
Plasmid pGAPDH-EGFP used for transformation of A. castellanii. The plasmid has an ampicillin resistance marker for propagation in Escherichia coli, neomycin resistance marker for selection in A. castellanii, and enhanced green fluorescent protein (EGFP) as a reporter gene. The neomycin resistance marker and EGFP are expressed from endogenous A. castellanii promoters. Expression of the resistance marker is driven by the TATA-box binding protein (TBP) promoter, while EGFP expression is driven by the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. Restriction sites used in this study are marked on the plasmid map